Corning 100 mL Trypan Blue Solution, 0.4% (w/v) in PBS, pH 7.5 ± 0.5 Rectangular Cover Glass Selenium aids in the detoxification

Selenium aids in the detoxification of free radicals as a cofactor for GSH synthetase

/Pk: 1 / PkPlate Format: 96-well Plate Feature: Standard Plate Color: Clear Well Bottom: Round Well Bottom Color: Clear Well Volume: 330 µL Recommended Medium Well Volume: 100 - 200 µL Recommended Working Volume: 75-200 µL Surface Treatment: TC-Treated Sterile: Yes Lids Included: Yes

external temperature controller

Streptomycin is an antibiotic which acts as a protein synthesis inhibitor

Viable cells exclude the dye

Corning 100 mL Trypan Blue Solution, 0.4% (w/v) in PBS, pH 7.5 ± 0.5 Rectangular Cover Glass Selenium aids in the detoxification0. 4% (w v) in normal saline (8. 1 g L NaCl with 0. 6 g L K2HPO4) Technical Advantage: Can be used for a variety cell counting methods requiring information on cell count and viability. Trypan Blue is the most common stain used in cell count and viability assays. Viable cells exclude the dye, while nonviable cells absorb the dye and appear blue. Cells should be in suspension as single cells in buffered saline before counting. Trypan blue can be used