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277 HO activates TRPM2 by targeting Glu829 and Arg845, producing NAD metabolites such as ADP-ribose, which opens calcium channels and induces cell death

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8 Functionally, the gut microbial metabolic profile of ALD patients undergoes profound changes, highlighted by the depletion of SCFAs (such as butyrate and propionate) and anti-inflammatory microbial metabolites (such as indole-3-propionic acid (IPA)), alongside increased production of microbially derived ethanol and endotoxins

Enzyme Activity Assays of the Antioxidant-Scavenging and Redox-Regulating Systems To measure enzyme activity, the oilseed rape plant root and leaf samples were homogenized with a 100-mM potassium phosphate buffer, pH 7.5, containing 1% polyvinylpyrrolidone-40, 1 mM of phenylmethylsulfonyl fluoride, and 2 mM of ethylenediaminetetraacetic acid (EDTA) (Lee et al., 2013)

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