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After removing sperms from the surface of the zona pellucida by repeated pipetting in porcine zygote medium-3 (PZM-3) (Yoshioka et al., 2002), a group of 10 presumable zygotes was cultured in 30 l drops of PZM-3 in 35-mm plastic dishes under paraffin oil at 38.5C in an atmosphere of 5% CO 2 and saturated humidity

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Detailed documentation will be crucial in establishing a timeline and potential causation

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During this process damage-associated molecular patterns mediate the recruitment and activation of dendritic cells, thereby facilitating T-cell infiltration and cytotoxicity