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Remarkably, nuclear receptor coactivator 4 (NCOA4), a key cytosolic autophagy receptor responsible for initiating ferritinophagy, mediates the degradation of ferritin, which subsequently increases intracellular Fe 2+ levels and lipid peroxidation, ultimately leading to ferroptosis [206, 207]

Alternatively, cell lysates containing the tagged bait protein and its potential interaction partners are directly added to the affinity resin

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