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Acta 1830 (5) (2013) 32173266

SDS-PAGE Western Blot Protein samples in 40 l of 1x electrophoresis sample buffer were boiled for 5 min at 95 C

Adapter ligation and PCR amplification were used to construct sequencing libraries, which underwent 150-bp paired-end sequencing on an Illumina NovaSeq

Obesity was associated with phylum-level changes in the GMB, with an increased proportion of the Bacillota phylum relative to Bacteriodota and a decreased alpha-diversity