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RT-PCR analysis Total RNA was isolated from cells using TRIzol reagent (Invitrogen, Carlsbad, CA, USA)

Combined Wnt/-catenin, Met, and CXCL12/CXCR4 signals characterize basal breast cancer and predict disease outcome

They include infection of hBECs of the BBB, transcytosis, and also infection of transmigration-competent immune cells which may enter the brain as a Trojan horse. A transwell 3D co-culture of HBMECs with astrocytes and monocytes showed how ZIKV-infected monocytes could cross the BBB to enter brain tissue (Bramley et al., via infected monocytes (Nottet et al., 1996