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For treatments that affected housekeeping gene expression, total protein normalization was performed using Bio-Rad stain-free SDS-PAGE gels, and protein loading was quantified based on total lane intensity using the ChemiDoc MP imaging system (Bio-Rad)

One week after mice surgery, cortical EEG signals were amplified, filtered, and digitalized with a resolution of 1000 Hz using a tethered data acquisition system (Bio-Signal Technologies) and continuously synchronized with infrared video for at least one hour and then analyzed off-line in the custom code and brainstorm based on Matlab 2021

Evidence for heterodimeric association of leukemia inhibitory factor (LIF) receptor and gp130 in the mouse uterus for LIF signaling during blastocyst implantation

Acute and subchronic toxicity studies in rodents generally report high LD values and no significant adverse effects at commonly used doses, indicating a wide safety margin

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