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Moreover, in transfected cells or in vitro assays using artificial membranes, the NEC stimulates uncontrolled vesicle formation, which is absent in infected cells 48,49,50

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In vitro release studies showed sustained release for over two weeks, best fitted by zero-order kinetics

As achieving calciumfree conditions is generally challenging and can create artifacts in proteoliposomes, another approach was employed to eliminate the calcium binding site by mutagenesis

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