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N2 and JK2729 worms were grown until the L4 stage on plates with heat-inactivated OP50, then transferred to 50 M FUdR-containing plates to prevent progeny development

Techniques such as fluorescence recovery after photobleaching and dynamic contrast-enhanced magnetic resonance imaging (MRI) have demonstrated that interstitial fluid generally moves at a relatively slow velocity, ranging from 0.1 to 10 m/s, depending on the species under consideration [214]

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