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Optimization of the fusion enzyme CrtZ ~ W for astaxanthin biosynthesis in C

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doi: 10.1093/molehr/gay045 91 CecchinoGNSeliEAlves da MottaELGarca-VelascoJA

Santos RX, Correia SC, Wang X, Perry G, Smith MA, Moreira PI, Zhu X (2010) Alzheimers disease: diverse aspects of mitochondrial malfunctioning

Breifly, the reaction consists of 1 mM 1-chloro-2,4-dinitrobenzene (CDNB), 5 mM glutathione (GSH) in 50 potassium phosphate buffer and 500 l of crude enzyme in 1 ml total volume

Multiple mechanisms are responsible for the anemia, but the main mechanisms are decreased RBC production due to suppression of erythropoiesis, reduced iron availability, and decreased RBC lifespan (extravascular hemolysis)