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RT-qPCR was performed on nine telomere sites (1q, 2q, 9p, 10q, 13q, 15q, 17p, XpYp, and XqYq) of siEWS-expressing cells with subtelomere-specific primers for each site and normalized to the reference gene GAPDH (Supplementary Table S2)

Bovine fibronectin (Thermo Fisher) was diluted to 20 g ml 1 in PBS and incubated overnight at 4 C in a flat-bottom 96-well plate (Greiner)

On This Page What is Glutathione Glutathione is a potent antioxidant that the body produces naturally from three amino acid starting materials: glycine, glutamine and cysteine