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This suppression is significantly less pronounced in GDAP1 overexpressing cells, compared with control cells (the graph represents 1 trial, 8 biological replicates)

These probes contain two cysteine residues, that are virtually completely reduced in the cytoplasm, but once exported into the periplasm, can form a disulfide bond, a process that can be monitored by fluorescence spectroscopy

However, when ROS levels surpass physiological thresholds, detrimental effects on heart cell function, as well as protein and lipid peroxidation, and DNA damage can occur [5]

Briefly, whole Drosophila were fixed in 4% PFA for 12 h at 4 C, then rinsed 3 PBS for 3 h at RT with gentle shaking, and then immersed in FlyClear Solution-1 (8% THEED (2,2,2,2- (Ethylenedinitrilo)-tetraethanol) (Sigma-Aldrich), 5% Triton X-100 (Sigma-Aldrich) and 25% Urea (Fisher Scientific, in PBS) for 14 days at 37 C, as described previously 26

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