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The reaction was allowed to proceed at RT and time points were removed at 0 and 30 min, quenched by mixing with 3x SDS-PAGE buffer (150 mM Tris-HCl, 20 vol% glycerol, 30 mM EDTA, 4% SDS and 4 vol% b-mercaptoethanol)

Oncogene 23 , 49754983 (2004)

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