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6M guanidine-HCl was diluted in protein-binding buffer [20 mM Tris (pH 7.5), 0.1M sodium chloride, 1 mM EDTA, 1 mM DTT, 10% (v/v) glycerol, 0.1% (v/v) Tween-20, 5% (w/v) nonfat dry milk]

DNA encoding the polypeptides of the present invention will typically be incorporated into DNA constructs capable of introduction to and expression in an in vitro cell culture

Western blot analysis (Cdc5 and Pgk1) Analysis of protein from cell lysates was performed as described previously 70

[DOI] [Google Scholar] 218.Asfour H.Z., Alhakamy N.A., Ahmed O.A.A., Fahmy U.A., Md S., El-Moselhy M.A., Rizg W.Y., Alghaith A.F., Eid B.G., Abdel-Naim A.B

Antioxidase-mimicking activities The O 2 and H 2 O 2 inhibition rates of ZMTP nanosheets were determined by using SOD assay kit and catalase assay kit at mild acidic solutions (pH = 6.6) following the manufacturers protocols