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To obtain purified LCs, 1 mL of the interstitial cells was loaded at the top of a 3660% (v/v

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hTrxR1 activity was measured using 5,5-dithiobis(2-nitrobenzoic acid) (DTNB) 22 as well as a hTrx1 mutant (C73S) 34 as substrates

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In a related analysis, GHK was noted for its ability to reverse, in vitro, an aggressive gene-expression signature associated with metastatic behavior in certain tumor-cell datasets a striking observation that is nonetheless purely computational/in-vitro and not a therapeutic claim, and one that should be read alongside the founding finding that the same peptide can stimulate growth in neoplastic cells

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