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Postnatal cortical neuron isolation and culture Postnatal cortical neurons were isolated following a protocol adapted from Brewer and Torricelli (2007) and the Miltenyi Biotec MACS protocol for the isolation and cultivation of neurons from adult mouse brain

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On day 4 and beyond, take 2 capsules twice daily, 30 to 60 minutes before your two largest meals (e.g., breakfast and lunch)