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As mentioned before, F 2 -IsoPs can be measured using chromatographic methods as well as commercially available immunoenzymatic tests

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However, many trials suffered from poorly considered experimental designs, and future studies need to pay attention to assessing several factors, such as the different types of SCFA (acetic acid, propionic acid, butyric acid, or mixtures thereof), the form of SCFA administration (fiber-rich diets or capsules containing sodium salts or inulin esters), the duration of the intervention, the route of administration (oral or intravenous infusion), and patient adherence

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Mature human IGF1 gene (residues 49118) was codon-optimized and inserted into a modified pET-28a vector encoding a SUMO-tag following the His6-tag at the N teriminal end