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three technical replicates each

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The figure displays the most plausible but minimum number of duplications

PCOS distinction from other similar disorders Due to the clinical presentation of patients with androgen excess being extremely similar in a common female condition, many national and international consensuses recommend that the diagnosis of PCOS should be systematically ruled out before the diagnosis of other special diseases associated with hyperandrogenism, signs of oligomenorrhea, and infertility [104]

This is where affinity tags come in they are peptides which do not naturally exist in most expression systems that can be added to the N or C terminus of your protein of interest

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