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ROS and live/dead cell staining TSPCs (5000 cells/cm) were cultured on a plastic petri dish and treated in duplicate with 500 M NAC and/or 500 M hydrogen peroxide (H 2 O 2 ) for 12 h [55, 56]

The linkage of NAD + metabolism to the internal clocks is firstly evidenced by that the NAD(P) + /NAD(P)H ratio modulates the DNA-binding activity of the core oscillators, such as CLOCK: BMAL1 and NPAS2: BMAL1 heterodimers

CAFs can recruit myeloid-derived immunosuppressive cells (MDSCs) and regulatory T cells (Tregs) by up-regulating the expression of chemokines CCL22 and CCL28, and further enhancing immunosuppressive TME (71)

Anabolic steroids are classified as Schedule 3 controlled drugs in the US and Class C drugs in the UK

10.1063/1.464397 17 De MarchM.TerdoslavichM.PolezS.GuarnacciaC.PoggianellaM.MarcelloA.et al (2022)