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This rigorous analytical validation verifies the precise amino acid sequence layouts, exact molecular weights, and correct spatial conformations while ensuring the total absence of residual synthesis reagents, truncated fragments, heavy metals, or raw contaminants

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250 of PBS containing human HGF (1.25ng) were incubated with 3 H-Hinge, the central dimerization domain of HGF, in the presence of varying concentrations of 6-AH analogs between 10 ~ ' 3 M to 10 "7 M (half-log dilutions) for 40 min at 37C