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RNA extraction and quantitative real-time PCR analysis All the tissues were harvested at indicated time points after APAP injection

Compared with the model group (inhibition 05.09%), the results indicated that all three polysaccharides inhibited the growth of HT29 cells in the xenograft model, with Dendrobium officinale polysaccharides (250 g/ml) exhibiting the most significant inhibitory effect on CRC (67.911.69%)

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