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Table 2 Potential risk DNA methylation changes associated with mechanisms of neurodegenerative diseases and their treatment strategies

Finally, data analysis was performed using the FlowJo software for quantifying the percentage of positive cells (uptake [%]) as well as the relative mean fluorescence intensity per cell (rMFI) according to: Evaluation of Transfection Efficiency via Flow Cytometry For calculating the siRNA gene silencing efficiency on eGFP-H1299 cells, 7,500 cells per well were seeded (for 48 h study) in 96-well plates which were allowed to attach overnight