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Molecular pathways documented in preclinical research include: (1) activation of nitric oxide (NO) synthesis via L-arginine/eNOS pathway, (2) transcriptional upregulation of VEGF-A and FGF-2 favouring angiogenesis, (3) modulation of the Egr-1 early transcription factor involved in fibroblast proliferation, (4) interaction with 5-HT2A and dopaminergic receptors in some behavioural models, (5) stabilisation of endothelial function via eNOS modulation and reduction of oxidative stress (8-OH-dG, MDA)

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The Recommended Dietary Allowance (RDA) for vitamin B12 varies by age and condition: Adults: 2.4 mcg/day Pregnant women: 2.6 mcg/day Breastfeeding women: 2.8 mcg/day However, injection dosages are significantly higher and vary depending on the individuals condition

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