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NF-B (p65) DNA-binding activity analysis NF-B (p65) DNA-binding activity in the nuclei of homogenized liver tissues or cultured cells was measured using ELISA kits (Cayman, MI, USA) in accordance with the manufacturers instructions

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Approval was granted by the Hacettepe University Animal Experimentations Local Ethics Board (approval number: 2013/7210)

However, in these studies, the analysis of the tongue muscle was approached qualitatively, yet without specific delineation of the particular region of the tongue analyzed

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