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Mechanistically, fructooligosaccharides can enrich Bifidobacterium in obese mice, activate the cAMP/PKA pathway in intestinal L cells, stimulate GLP-2 secretion, enhance tight-junction protein assembly in the colonic epithelium, improve barrier function, and reduce systemic inflammation (191)

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Ng et al., 2015

Numerous previous studies have established a close association between renal injury and the occurrence of ferroptosis

A42 deposition also decreases the level of antiapoptotic protein B cell lymphoma 2 (Bcl-2), upregulates the pro-apoptotic protein Bax, and increases activities of apoptosis-related proteins caspase-3, caspase-9 and cytochrome c (Cytc) in SH-SY5Y cells, ultimately causing apoptosis and neurite degeneration [70, 79, 81]

In the case of mass spectrometry (MS)- based metabolomics, several strategies are use, e.g., desorption electrospray ionization mass spectrometry (DESI-MS) or flow injection analysis-MS (FIA-MS), or in combination with separation techniques such as gas chromatography-mass spectrometry (GC-MS), liquid chromatography-mass spectrometry (LC-MS), or capillary electrophoresis-MS (CE-MS), to increase separation and better identification and quantitation of the metabolites present in a cell extract ( In order to maximize the effectiveness of in vitro metabolomic studies for hepatotoxicity, it is important to have a comprehensive coverage of the cells metabolome