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A 1 mL aliquot of binding/wash solution was added to the tube, mixed by gentle pipetting to perform magnetic separation, the supernatant was discarded and the beads were washed twice

Moreover, -phosphatase treatment of lysates from nocodazole-treated HEK-293T cells markedly enhanced the Sororin-Pds5B co-immunoprecipitation, without affecting interactions with RAD21-N103 or SMC3-HD (Fig

doi: 10.1016/j.toxlet.2020.11.002 114

Approximately 80% of the injected fluid is likely to be removed within eight hours of delivery

JAHA 6, e004328

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