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For homologous recombination mediated BAC deletions, ~1-kilo-base pair (kb) sized 5 and 3 arms were amplified via polymerase chain reaction (PCR) and cloned into the pGEM - T - easy vector (Promega) and in between these arms, an Ampicillin (Amp)-resistant (r) gene cassette or a Kanamycin (Kan)-r cassette put between two FRT sites was subcloned

Importantly, HER-2/neu overexpression is a clinically relevant target in human breast cancers, making this model highly translational for cancer risk mitigation in susceptible populations

I feel better than I did before kids and I wish I did this sooner

Kassim M, Achoui M, Mustafa MR, Mohd MA, Yusoff KM, et al

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PMC 4402723