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For GST fusion proteins, cells were lysed by sonication in Buffer A (50 mM Tris-HCl, pH 8.0, 300 mM NaCl, 1 mM EDTA, 0.5% Triton X-100, 1 mM DTT, 1 mM phenylmethylsulfonyl fluoride/PMSF))

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PMCID: PMC111149 Xie Y et al (2012) H2 enhances Arabidopsis Salt Tolerance by manipulating ZAT10/12-Mediated antioxidant Defence and Controlling Sodium Exclusion

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2 Materials and methods 2.1 Plant material The plant material used in this study consists of bark from the trunk of F

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