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Ultra-high performance liquid chromatography (UPLC) was performed using a 1.7 m particle, 2.1 mm 100 mm, CSH C18 Column (Waters, Milford, MA, United States) coupled to a quadrupole TOF mass spectrometer (AB SCIEX, TripleTOF 5600) operated in information-dependent MS/MS acquisition mode

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Clean the injection site area and vial top with alcohol swabs