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Gallagher RC, Van Hove JLK, Scharer G, Hyland K, Plecko B, Waters PJ, et al

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For lipid-supported respiration, oxygen consumption was measured at steady state in the presence of 0.01 mM blebbistatin, 0.5 mM malate, 0.5 mM CoA, 5 mM carnitine, and 0.02 mM palmitoyl-CoA

Briefly, cells were rinsed with PM buffer (20 mM Na-phosphate, 1 mM MgSO 4 , pH 7.4) and fixed with 4% paraformaldehyde in PM buffer

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