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It is now quite a common technique

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For sequential image acquisition, the following imaging parameters were used: excitation 405 nm (emission 450/50 nm) for Hoechst and excitation 561 nm (emission 600/50 nm) for Alexa Fluor 568 with 50 ms and 200 ms exposure time, respectively

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Spain also led in international collaborations, with CIBERCentro de Investigacin Biomdica en Red and Harvard University being the most prominent collaboration centers

We also have an infographic that covers all the lasted B12 recommendations.