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Next, the samples were centrifuged for 10 min at a speed of 6000 revolutions per minute [rpm]

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Statements Author contributions The author confirms being the sole contributor to this work and has approved it for publication

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By performing gene set enrichment analysis (GSEA) limited to human-derived reads, we confirmed the upregulation of senescence and IFN- response signatures on palbociclib treatment and their downregulation when compared with palbociclib + RSL3-treated mice (Fig

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Then, the supernatants were transferred to new 1.5-mL microcentrifuge tubes, and 9 L of an RNase mixture of equal amounts of pancreatic ribonuclease (RNase A, 10 mg/mL, Sigma-Aldrich) and ribonuclease T1 (RNase T1, 50 KU, Sigma-Aldrich) were added to the samples

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