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Following primary antibody incubation, cells were treated with goat anti-rabbit IgG H&L Alexa Fluor 488 (ab150077, Abcam), donkey anti-goat IgG H&L Alexa Fluor 647 (ab150131, Abcam) and DAPI (Invitrogen) for 1 hour in the dark at room temperature

Recently, a transient-state characterization of Sm TGR U597C reductive half-reaction was completed (Smith et al., 2023)

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