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Fumarate, an allosteric activator, can largely increase ME2 activity by lowering K 0.5,malate and K m,NAD, thus enhancing the affinity of ME2 for its substrates (Fig

The amplified promoter regions were cloned into pCAMBIA1304 vector between BamHI and BglII restriction enzyme (RE) sites to generate AtNRAMP3pro :: GUS and AtNRAMP4pro :: GUS constructs and between BamHI and SpeI to produce AtPIC1pro :: GUS construct