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According to the results of our pilot experiments (data not shown), the HSA concentration, the ratio of GSH to HSA, the temperature of the reduction reaction, the ratio of ATRA to HSA, the ratio of ATO and ATRA, the injection speed of ATRA solution, and the volume ratio of ethanol and water were set to 4%, 38.5%, 40 , 3%, 12.7%, 1.5 mL min 1 , and 5:1, respectively

Keep the metered spray pump clean and refrigerated at all times when not in use to minimize degradation of the peptide

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The conditions, such as a 75 mM sodium phosphate buffer (pH 3.0) with 1.7 mM 1-octanesulfonic acid, 25 M EDTA, 10% methanol, and a flow rate of 1 mL/min, are optimized for precision and sensitivity, ensuring accurate neurotransmitter profiling at a column temperature of 25C

The visible channel allows for a very large dynamic range of illumination (approximately 10 7 )

Lowering the pH causes a shift toward the tetrameric form