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This increase, in turn, activates the NLRP3 inflammasome, which subsequently triggers the activation of caspase-1

Serum samples were collected on day 28 and stored at 35C, as previously described ( 2.5 Hybridoma fusion, screening, and mAbs purification The spleens of selected mice were used to isolate SCMS- or MetE-specific mAbs as previously described ( 2.6 ELISA The antigen specificity of the immunized mice sera and of the hybridoma supernatants was analyzed by ELISA, as previously described ( 2.7 SDS-PAGE and Western blot analysis SDS-PAGE and WB were carried out to analyze recombinant proteins and SCMS, as previously described ( Human sera were analyzed by WB after SDS-PAGE of rMetE: blotted membranes were incubated with sera diluted 1:100 and detected using HRP-conjugated goat anti-human IgG (Sigma-Aldrich) and signals were detected by Crescendo Western HRP chemiluminescent substrate

Kirmit et al

Favero G, Bonomini F, Franco C, Rezzani R

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Its primary role here is to act as a chemical buffer to protect the BPC-157 peptide, not to provide a significant physiological effect of its own