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Preparation of algal extracts The extraction of air-dried powder from Chlorella vulgaris , Nannochloropsis salina and Arthrospira platensis algae was performed using 80% methanol [PIOCHEM], following the method described by 28

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For the biotinylation an appropriate volume of 10 mM Sulfo-NHS- Biotin solution (Pierce Chemical Co., Rockford, IL) solution was incubated with 200 ml of each BALF for 30 minutes at room temperature with agitation