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Chromatographic separation was achieved on a Platinum EPS C18 100 A 3 u (length: 53 mm, internal diameter: 7 mm, maximum pressure: 5000 PSIG) analytical column (Alltech Associates, Inc., Deerfield, Ill.) at ambient temperature with a flow rate of the mobile phase maintained at 1 ml/min

Electrolytes (sodium, potassium, magnesium) prevent dehydration-related symptoms during reduced food intake