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Add 75 L of R2 reagent containing catechol (20.0 mmol/L), bovine serum albumin (1.0 g/L), and sodium azide (0.5 g/L) to the sample, incubate at 37C for 5 min, measure the absorbance A2, calculate the difference in absorbance A = A2-A1, sample concentration = sample ( A) standard concentration/standard ( A)

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