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Common mistakes include: Using non-sterile water Assuming BAC Water and Sterile Water are identical Reusing opened Sterile Water repeatedly Improper peptide refrigeration after reconstitution Failing to maintain sterile handling procedures Research discussions online frequently identify diluent confusion as one of the most common beginner peptide handling problems

PMID: 26520732

10.1016/j.jsbmb.2021.105992 142 XuB.DaiW.LiuL.HanH.ZhangJ.DuX.et al (2022)

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By ending the incubation for 4 h, the MTT solution and RPMI medium were aspirated, and DMSO (150 L) was added to dissolve the formazan crystals