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One in vivo comparison of GHK versus GHK-Cu in rat experimental wounds found that GHK alone had no effect, and only the copper-bound GHK-Cu form produced a statistically significant increase in collagen accumulation evidence that the copper ion itself, not the peptide backbone alone, may be responsible for much of the collagen-stimulating activity

doi: 10.1016/j.psyneuen.2010.03.010 117 HalliwellB

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HTMCs were trypsinized and resuspended in culture medium at a density of 2.2 10 6 cells/ml