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IGF1R, as a transmembrane glycoprotein and stress-receptor, could feel the stress outside and send these stress signals to intracellular, activating downstream pathways to regulate cell proliferation, migration, and chemotherapeutic resistance [14]

Cell lysates and basilar supernatants were run in 4%12% sodium dodecyl sulphatepolyacrylamide (SDS-PAGE) gels (Invitrogen) and transferred onto polyvinylidene difluoride membrane (Invitrogen)

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Fame RM, Kalugin PN, Petrova B, Xu H, Soden PA, Shipley FB, Dani N, Grant B, Pragana A, Head JP, et al