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Pyruvate kinase ( PKM ) regulates the final rate-limiting step of glycolysis, which catalyzes the generation of two molecules of pyruvate and two molecules of adenosine triphosphate (ATP

Many perimenopausal women are progesterone-deficient (due to anovulatory cycles) years before their estrogen significantly declines, and low progesterone is a primary driver of mood instability, sleep disruption, and anxiety in early perimenopause

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Depending on the plate layout, either LPS (1 g/ml) or ConA, (2.5 g/ml, Sigma-Aldrich, Steinheim, Germany) was added in four replicates per cow in addition to four unstimulated wells (with Seahorse medium for volume compensation)

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The administration of the study drug will be completed through the Research Pharmacy Office located at the Kaye Edmonton Clinic