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doi: 10.1038/d41586-019-02340-x 48 LiFTangYSongBYuMLiQZhangCet al

Preventing complications begins long before the first injection

Microinjection and selection and confirmation of transgenic zebrafish To efficiently excise and integrate the DNA fragment surrounded by the Tol2 element transposon into the genome, we employed co-injection of in vitro-transcribed Tol2 transposase mRNA as described earlier 28