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5 Disruption of the neuroendocrine network and the local biochemical microenvironment The pathological microenvironment of Chronic Prostatitis/Chronic Pelvic Pain Syndrome (CP/CPPS) involves not only inflammatory-immune crosstalk and oxidative stress, but alterations in local biochemical components and dysregulation of the neuroendocrine network also represent crucial links in the impairment of sperm development

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In most of the responses to stress, the plant promotes an increase in reactive oxygen species (ROS) (Dias et al., 2011

The primary antibody was incubated over night at 4 C and the secondary biotinylated antibody mediated horseradish peroxidase (HRP)-driven 3,3-diaminobenzidine (DAB, DAKO, K3468) turnover, which resulted n brown labeling of immunoreactive cells