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For lysis, cell pellets were resuspended in 1 mL of buffer A (20 mM HEPES/KOH pH 7.5, 150 mM KOAc, 10 mM MgCl, 1 mM DTT, and cOmplete Protease Inhibitor [1 tablet/50 mL, Roche]), homogenized using a 7-mL Dounce glass homogenizer (B pestle, 20 strokes), and centrifuged twice at 12,000 g for 15 min at 4 C

For cell colony formation assay, cells were seeded in 6-well plated at a density of 12 10 3 cells per well

Lochhead JJ, Thorne RG

The cycling different peptides guide addresses the specific considerations for rotating between compounds and managing transitions between cycles