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Key assays included senescence-associated beta-galactosidase (SA--gal) activity, RT-PCR, western blotting, immunofluorescence, live-cell confocal imaging with fluorescent probes, flow cytometry and mitochondrial respiration analysis

The model suggests that the two tryptophans (W47, W50) dock into a hydrophobic cavity located on the opposite side of the typical zf-UBP binding site for the di-Gly motif of ubiquitin (Fig