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Beyond just a tight fortress: contribution of stroma to epithelial-mesenchymal transition in pancreatic cancer

Glutathione, Oxidized (GSSG) is the oxidized version of the naturally occurring and very important antioxidant glutathione (GSH)

E.CaroR

The epithelial cells reported to have a great metabolic rate and divide rapidly which has been considered to be a potent factor responsible for the oxidation of DNA

Samples were reduced with DTT (5 L of 200 mM stock in H 2 O, 10 mM final concentration) and incubated at 65 C for 15 min, then alkylated with iodoacetamide (5 L of 400 mM stock in H 2 O, 20 mM final concentration) and shaken at 37 C for 30 min in the dark

Binding to antioxidant response elements (AREs) induces the transcription of genes regulated by AREs, which initiates the expression of genes encoding cytoprotective enzymes, such as antioxidative stress proteins, especially antioxidant enzymes ( Figure 2 The antioxidant response is also modulated by signaling pathways mediated by adenosine 5'-monophosphate-activated protein kinase (AMPK)