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Real-time quantitative PCR Following drug treatments, total RNA was extracted from THP-1 cells using Trizol reagent (Biosharp, BS259A) and subsequently reverse transcribed into cDNA using the RevertAid First Strand cDNA Synthesis Kit (Thermo Fisher Scientific, K1691)

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meditteranei , a perchlorate-reducing microorganism (Oren et al., 2014), also demonstrated the enzymes ability to reduce perchlorate (Martnez-Espinosa et al., 2015)

Alternatively, a detailed proteomic study on tomato fruit confirmed the implication of ASC- and GSH-related proteins in response to this abiotic stress (Marmiroli et al., 2017b)

However, the recombinant constructs of Gsh2-Gsh1 and Pro1-GshB with a flexible linker have not been exploited for improving intracellular GSH synthesis until now