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In each well of a poly-L-lysine coated 96-well plate (clear, GreinerBio, Kremsmnster, Austria), approximately 4000 cells were seeded in 100 l Dulbeccos Modified Eagle Medium (DMEM) containing 10% foetal calf serum (FCS) and 1% penicillin and streptomycin (P/S)

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Also it improves the blood circulation in your body, reduces lactic acid levels in the blood which furthur reduces blood pressure